gamma globin (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
gamma globin
Gamma Globin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+globin/Hemoglobin+%CE%B3+Antibody/pmc12356964-425-38-41
Average 93 stars, based on 70 article reviews
Gamma Globin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+globin/Hemoglobin+%CE%B3+Antibody/pmc12356964-425-38-41
Average 93 stars, based on 70 article reviews
gamma globin - by Bioz Stars,
2026-09
93/100 stars
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SDS Page:Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4-20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4–20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2 um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Article Title: Deficiency of ribosomal protein S26, which is mutated in a subset of patients with Diamond Blackfan anemia, impairs erythroid differentiation Article Snippet: .. Proteins were separated by electrophoresis on a 12% SDS-PAGE gel, transferred to nitrocellulose membranes and incubated with antibodies specific for RPS26 (#SAB1302676, Sigma-Aldrich), alpha globin (#sc-514378, Santa Cruz Biotechnology), Electrophoresis:Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4-20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4–20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2 um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Article Title: Deficiency of ribosomal protein S26, which is mutated in a subset of patients with Diamond Blackfan anemia, impairs erythroid differentiation Article Snippet: .. Proteins were separated by electrophoresis on a 12% SDS-PAGE gel, transferred to nitrocellulose membranes and incubated with antibodies specific for RPS26 (#SAB1302676, Sigma-Aldrich), alpha globin (#sc-514378, Santa Cruz Biotechnology), Membrane:Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4-20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4–20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2 um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Staining:Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4-20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Article Title: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Article Snippet: Cells were resuspended in RIPA buffer (Cell Signaling Technology), vortexed, sonicated, and frozen. .. Following 4–20% gradient SDS page gel (Biorad) electrophoresis, proteins were transferred to a 0.2 um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919, Cell Signaling Technology), Beta globin (sc-21757, Santa Cruz), Protein Extraction:Article Title: A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells Article Snippet: .. Protein extraction, immunoblotting and detection of immunocomplexes were performed as previously detailed (Sivalingam et al., 2018) with the following primary antibodies [1:800 diluted beta-globin (Santa Cruz, SC-21757), 1:400 diluted Article Title: A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells Article Snippet: .. Protein extraction, immunoblotting and detection of immunocomplexes were performed as previously detailed (Sivalingam et al., 2018) with the following primary antibodies [1:800 diluted beta-globin (Santa Cruz, SC-21757), 1:400 diluted Western Blot:Article Title: A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells Article Snippet: .. Protein extraction, immunoblotting and detection of immunocomplexes were performed as previously detailed (Sivalingam et al., 2018) with the following primary antibodies [1:800 diluted beta-globin (Santa Cruz, SC-21757), 1:400 diluted Article Title: A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells Article Snippet: .. Protein extraction, immunoblotting and detection of immunocomplexes were performed as previously detailed (Sivalingam et al., 2018) with the following primary antibodies [1:800 diluted beta-globin (Santa Cruz, SC-21757), 1:400 diluted Incubation:Article Title: Deficiency of ribosomal protein S26, which is mutated in a subset of patients with Diamond Blackfan anemia, impairs erythroid differentiation Article Snippet: .. Proteins were separated by electrophoresis on a 12% SDS-PAGE gel, transferred to nitrocellulose membranes and incubated with antibodies specific for RPS26 (#SAB1302676, Sigma-Aldrich), alpha globin (#sc-514378, Santa Cruz Biotechnology), |
![Use of pomalidomide as a positive control for fetal hemoglobin (HbF) induction. (A) Primary erythroid progenitors derived from a healthy donor were treated with 10 μM pomalidomide under proliferation conditions for 5 days and then switched to differentiation conditions. Samples for quantitative reverse‐transcription polymerase chain reaction analysis of γ‐globin expression were taken at Days 0, 3, 5 (proliferation conditions) and Days 8 and 10 (differentiation conditions, red font). Treatment: gray: control; blue: dimethyl sulfoxide (DMSO) (solvent control), purple: 10 μM pomalidomide. N = 3 independent cultures; error bars indicate standard deviations. (B) Western blot analysis of β‐globin and γ‐globin expression of DMSO and pomalidomide (Pom) treated samples shown in (A) HbF levels were measured by high‐performance liquid chromatography (HPLC) at Day 10 of culture. (C) Primary erythroid progenitors derived from an sickle cell disease patient were treated with 10 μM pomalidomide under proliferation conditions for 5 days and then switched to differentiation conditions. Other details are as for panel A. (D) Western blot analysis of β‐globin and γ‐globin expression of DMSO and pomalidomide (Pom) treated samples shown in (C). HbF levels could not be measured by HPLC at Day 10 of culture due to insufficient number of cells. (E) HUDEP2 and Aγ‐HiBiT reporter cells were treated with 10 μM pomalidomide under proliferation conditions for 5 days and then switched to differentiation conditions. H = HUDEP2 cells; R = Aγ‐HiBiT reporter cells. Other details are as for panel A. (F) HbF levels were measured by HPLC at Day 10 of culture of HUDEP2 cells and Aγ‐HiBiT reporter cells. See panel (E) for other details. (G) The HiBiT lytic assay reported Aγ‐HiBiT induction after the Aγ‐HiBiT reporter cells had been exposed to 10 μM pomalidomide (see E, F). (H) Western blot analysis of β‐globin and γ‐globin expression of control, DMSO, and pomalidomide (Pom)‐treated HUDEP2 cells (see E, F). (I) Western blot analysis of β‐globin and Aγ‐HiBiT expression of control, DMSO, and pomalidomide (Pom)‐treated Aγ‐HiBiT reporter cells (see E, F). (J, K) Viability and HiBiT lytic assays of Aγ‐HiBiT reporter cells treated for 72 h with pomalidomide at various concentrations showed a maximum 6.9‐fold induction of Aγ‐HiBiT signal (EC 50 = 0.9 µM), but no toxicity up to 50 µM pomalidomide. Error bars indicate the standard deviation of N = 3 independent cultures. (L) Although differentiation alone increased globin expression and thus the Aγ‐HiBiT signal, the addition of pomalidomide to the differentiation medium resulted in an additional 7.7‐fold increase in Aγ‐HiBiT signals. Error bars indicate the standard deviation of N = 3 independent cultures. (M, N) A toxic compound (no surviving cells at 50 µM, CC 50 = 1.7 µM) did not induce Aγ‐HiBiT expression. Error bars indicate the standard deviation of N = 3 independent cultures. (O) The Aγ‐HiBiT reporter cells were sensitive to the solvent DMSO (CC 50 = 0.55% [v/v]).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2795/pmc11302795/pmc11302795__HEM3-8-e139-g006.jpg)